Williams has not played a competitive match since losing to Ajla Tomljanovic in the third round of the 2022 US Open.
The 23-time Grand Slam winner said she will be competing in the doubles event at Queen's Club alongside Canadian teenager Victoria Mboko.
"Queen's Club feels like the perfect place to begin this next chapter," said Williams.
"Grass has given me some of the most meaningful moments of my career, and I'm excited to be back competing on one of the sport's most iconic stages."
Tournament director Laura Robson added: "Serena Williams is one of the greatest athletes the world has ever seen, and we're delighted that she will be making her return to tennis at the LTA's HSBC Championships.
"Women's tennis made a historic return to The Queen's Club last year, and now we have an icon of the game stepping back on to court at this prestigious venue - it's very exciting for the tournament and the fans."
The comeback follows Williams' re-entry into the testing program of the International Tennis Integrity Agency (ITIA), a requirement for players returning to competition.