The Canadian took to Instagram to announce the end to a career which has been defined by great achievements but tainted by inconsistency and injury.
"You'll know when it's time. For me, it's now. Ending where it all started: Montreal," Bouchard announced, alongside a variety of nostalgic pictures.
As a junior, Bouchard lifted the Wimbledon girls' singles title in 2012, winning the WTA's Newcomer of the Year award at the end of 2013.
She later went on to reach the final of the grass-court Grand Slam in 2014, losing out to Petra Kvitova 6-3 6-0 in just 55 minutes.
As a result of the performance, she went on to reach a career-high ranking of World No 5 in October that year.
But despite her early success, Bouchard's career was hampered by injuries and a dip in form that saw her slide down the rankings. A concussion sustained during a fall at the 2015 US Open marked the beginning of a challenging period, and she was never able to recapture her peak performance.
Ultimately, the Canadian managed just one win on the WTA Tour, the Nuremberg Cup in 2014.
The Montreal event, where she will hang up her racket, will take place from July 27 - Aug 7.
"Few athletes have left as profound a mark on Canadian tennis as Genie [Bouchard] has throughout her extraordinary career," stated Gavin Ziv, Chief Executive Officer of Tennis Canada.
"As one of the very first players to ever be part of the National Tennis Centre presented by Rogers in Montreal, she achieved things that many thought would be impossible for Canadian players.
"Her career highlights, which include a Wimbledon final appearance and a Billie Jean King Cup championship, are nothing short of extraordinary and were a true catalyst for the development of tennis in our country.
"She's been and still is an incredible ambassador for our sport and, on behalf of Tennis Canada, I want to thank her for everything she's done. We wish her continued success in the next chapter of her career."
"She has been one of the most important figures in the history of our sport in Canada and a trailblazer who redefined what Canadian tennis could be," remarked Valerie Tetreault, National Bank Open's tournament director.
"We are proud of everything she has done, as a player and role model, and we can't wait to see her in action one last time at IGA Stadium this summer."